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1.
Regulation of insulin receptor function 总被引:1,自引:0,他引:1
Youngren JF 《Cellular and molecular life sciences : CMLS》2007,64(7-8):873-891
Resistance to the biological actions of insulin contributes to the development of type 2 diabetes and risk of cardiovascular
disease. A reduced biological response to insulin by tissues results from an impairment in the cascade of phosphorylation
events within cells that regulate the activity of enzymes comprising the insulin signaling pathway. In most models of insulin
resistance, there is evidence that this decrement in insulin signaling begins with either the activation or substrate kinase
activity of the insulin receptor (IR), which is the only component of the pathway that is unique to insulin action. Activation
of the IR can be impaired by post-translational modifications of the protein involving serine phosphorylation, or by binding
to inhibiting proteins such as PC-1 or members of the SOCS or Grb protein families. The impact of these processes on the conformational
changes and phosphorylation events required for full signaling activity, as well as the role of these mechanisms in human
disease, is reviewed in this article.
Received 3 August 2006; received after revision 1 December 2006; accepted 8 January 2007 相似文献
2.
《科学通报(英文版)》1998,43(5):363-363
During the long period of time when people have been seeking for an effective therapeutic method for PD, the gene therapy has shown greater and greater advantages over other methods. It can be performed mainly in two ways: ex vivo and in vivo. With the former, TH gene as well as some neurotrophic factor genes (such as GDNF, BNDF genes) can be invited to some cell lines or primary cells thus forming engineered cells and then implanting them into brain. While with the latter, viral vectors including HSV-1, Ad, AAV that can be utilized to construct recombinant viruses, or non-virus vectors can be used to delived DNA into brain directly. The present review summarizes the recent research advances in the gene therapy for PD, and it is reasonable for us to predict a notable progress in prevention and treatment for PD in the next decade. 相似文献
3.
用辣根过氧化物酶(HRP)单酶先后标记Fos和酪氨酸羟化酶(TH),用二氨基联苯胺(DAB)增强剂和DAB分别呈色;以及同时用碱性磷酸酶(AP)和HRP双酶分别标记TH和Fos,再分别用5-溴-4-氯-3-吲哚磷酸盐(BCIP)和氯化硝基四氮唑蓝(NBT)对TH和DAB对Fos呈色,比较免疫双标时两种不同酶标记显色方法... 相似文献
4.
通过改进了的催化剂、碱含量及溶剂体系等条件,合成了N-磷酰酪氨酸,并在所拟定的实验条件下研究了常用的几种二烷基亚磷酸酯对酪氨酸的反应性。 相似文献
5.
D. P. Keogh M. J. Mitchell J. R. Crooks S. L. Smith 《Cellular and molecular life sciences : CMLS》1992,48(1):39-41
The adenylate cyclase activator forskolin and its pharmacologically inactive derivative 1,9-dideoxyforskolin were found to inhibit in a dose-dependent fashion the ecdysone 20-monooxygenase activity associated with wandering stage larvae ofDrosophila melanogaster and fat body and midgut from last instar larvae of the tobacco hornworm,Manduca sexta. The concentrations of these labdane diterpenes required to elicit a 50% inhibition of the cytochrome P-450 dependent steroid hydroxylase activity in the insect tissues ranged from approximately 5×10–6 to 5×10–4 M. 相似文献
6.
Thyroid hormone controls carnitine status through modifications of γ-butyrobetaine hydroxylase activity and gene expression 总被引:1,自引:0,他引:1
Galland S Georges B Le Borgne F Conductier G Dias JV Demarquoy J 《Cellular and molecular life sciences : CMLS》2002,59(3):540-545
The carnitine system plays a key role in β-oxidation of long-chain fatty acids by permitting their transport into the mitochondrial
matrix. The effects of hypothyroidism and hyperthyroidism were studied on γ-butyrobetaine hydroxylase (BBH), the enzyme responsible
for carnitine biosynthesis in the rat. In rat liver, BBH activity was decreased in the hypothyroid state and increased in
hyperthyroid animals. The modifications in BBH activity correlated with changes in the enzyme Vmax values. These changes were
shown to be related to hepatic BBH mRNA abundance. Thyroid hormones are known to interact with lipid metabolism, in particular
by increasing long-chain fatty acid oxidation through activation of carnitine-dependent fatty acid import into mitochondria.
Our study showed that thyroid hormones also increased carnitine bioavailability.
Received 23 October 2001; received after revision 11 January 2002; accepted 15 January 2002 相似文献
7.
Summary Plasma concentrations of gonadotropin, prolactin and hypothalamic tyrosine hydroxylase (TH) activity were measured in ovariectomized rats treated with aminooxyacetic acid (AOAA), a drug which elevates brain GABA levels. Hypothalamic TH activity was significantly increased with a significant decrease in prolactin (Prl) release. Plasma levels of gonadotropins were not modified by AOAA. These results support an inhibitory action of GABA on Prl release possibly mediated through hypothalamic dopamine.Supported by grants from Indian Council of Medical Research (ICMR), New Delhi. RIA kits for the estimation of LH, FSH and Prl were kindly supplied by Dr A.F. Parlow, NIAMDD-NIH, Bethesda, Maryland, USA. GNB is a UGC research fellow. 相似文献
8.
王心良 《西华师范大学学报(哲学社会科学版)》1998,19(2):185-188
标题化合物,一般以叔丁氧羰基肼和L-酪氨酸通过叠氮法获得,但常得到N-BOC-L-Tyr和N,O-diBOC-L-Yyr的混合物,极能分离纯化,作者研究在导入BOC后,以NH3为选择脱保护试剂,并采用彻底酸化试剂避免了原酸化步骤中胶化的问题,使双保护产物转化成目标产物,产品纯净,收率高。 相似文献
9.
吴杰 《哈尔滨师范大学自然科学学报》2013,(5):57-60
构建酪氨酸蛋白激酶Src真核表达载体,并检测其在HEK293T细胞中的表达.根据Genebank(GI:4574718)提供的大鼠Src的cDNA序列设计特异性引物,以大鼠海马总RNA为模板,采用RT-PCR方法扩增Src的目的基因,然后克隆至真核表达载体pcDNA3.1.将筛选的阳性重组子转染入HEK293T细胞系,免疫印迹检测Src的蛋白表达水平,经限制性内切酶双酶切及测序分析,扩增的Src的cDNA成功连接入pcDNA3.1,并且序列与Genebank中一致.免疫印迹结果表明,构建的Src-pcDNA3.1可在HEK293T细胞中表达.成功构建了Src-pcDNA3.1真核表达载体,并且可在HEK293T细胞中高效表达. 相似文献
10.